Sign In | Join Free | My xpandrally.com
xpandrally.com
Products
Search by Category
Home > General Assay & Diagnostic Apparatuses >

Human Optineurin (OPTN) ELISA Kit

Categories Human ELISA Kit
Place of Origin: China
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
  • Haven't found right suppliers
  • Our buyer assistants can help you find the most suitable, 100% reliable suppliers from China.
  • And this service is free of charge.
  • we have buyer assistants who speak English, French, Spanish......and we are ready to help you anytime!
  • Submit Buying Request
    • Product Details
    • Company Profile

    Human Optineurin (OPTN) ELISA Kit

    Human Optineurin (OPTN) ELISA Kit

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human Optineurin (OPTN) ELISA Kit
    Method:Sandwich
    Synonyms:

    NRP; FIP2; GLC1E; HIP7; HYPL; TFIIIA-INTP; Glaucoma 1,Open Angle,E(Adult-Onset); Huntingtin-interacting protein 7; Optic neuropathy-inducing protein; E3-14.7K-interacting protein

    Detection range:78.125-5000pg/mL
    Reactivity:OPTN
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-OPTN-Hu (traditional)DLR-OPTN-Hu (ready-to-use)
    Assay length1-4.5Hours1-3.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    Introduction
    ItemStandardTest
    Description

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of OPTN in human tissue homogenates or other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120μLDetection Solution A 1×12mL
    Detection Reagent B 1×120μLDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
    Instruction manual 1Instruction manual 1
    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to OPTN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to OPTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

    After TMB substrate solution is added, only those wells that contain OPTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.

    The concentration of OPTN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Recovery

    Matrices listed below were spiked with certain level of recombinant OPTN and the recovery rates were calculated by comparing the measured value to the expected amount of OPTN in samples.

    MatrixRecovery range (%)Average(%)
    serum(n=5)82-10292
    EDTA plasma(n=5)91-9894
    heparin plasma(n=5)86-10193
    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPTN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample1:21:41:81:16
    serum(n=5)82-96%83-98%81-99%93-101%
    EDTA plasma(n=5)88-101%86-95%90-102%80-93%
    heparin plasma(n=5)80-91%82-90%95-104%

    79-95%


    Product Tags:

    human vegf elisa

      

    human vegf elisa

      
    Quality Human Optineurin (OPTN) ELISA Kit for sale
    Send your message to this supplier
     
    From:
    To: Wuxi Donglin Sci & Tech Development Co.,Ltd.
    Subject:
    Message:
    Characters Remaining: (0/3000)
     
    Find Similar Products By Category:
    Inquiry Cart 0