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Human Alpha-2-Microglobulin Like Protein 1 (a2ML1) ELISA Kit

Categories Human ELISA Kit
Place of Origin: China
Brand Name: DLdevelop
Certification: ISO9001:2015, CE
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
Model Number: DL-a2ML1-Hu
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    Human Alpha-2-Microglobulin Like Protein 1 (a2ML1) ELISA Kit

    Human Alpha-2-Microglobulin Like Protein 1 (a2ML1) ELISA Kit

    two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.
    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human Alpha-2-Microglobulin Like Protein 1 (a2ML1) ELISA Kit
    Method:Sandwich
    Synonyms:

    CPAMD9; C3 And PZP-Like,Alpha-2-Macroglobulin Domain Containing 9

    Detection range:0.156-10ng/mL
    Reactivity:a2ML1
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-a2ML1-Hu (traditional)DLR-a2ML1-Hu (ready-to-use)
    Assay length1-4.5Hours1-3.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    1. Overview
    Other names:CPAMD9; C3 And PZP-Like,Alpha-2-Macroglobulin Domain Containing 9
    Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.
    Sequence:
    MWAQLLLGML ALSPAIAEEL PNYLVTLPAR LNFPSVQKVC LDLSPGYSDV
    KFTVTLETKD KTQKLLEYSG LKKRHLHCIS FLVPPPAGGT EEVATIRVSG
    VGNNISFEEK KKVLIQRQGN GTFVQTDKPL YTPGQQVYFR IVTMDSNFVP
    VNDKYSMVEL QDPNSNRIAQ WLEVVPEQGI VDLSFQLAPE AMLGTYTVAV
    AEGKTFGTFS VEEYVLPKFK VEVVEPKELS TVQESFLVKI CCRYTYGKPM
    LGAVQVSVCQ KANTYWYREV EREQLPDKCR NLSGQTDKTG CFSAPVDMAT
    FDLIGYAYSH QINIVATVVE EGTGVEANAT QNIYISPQMG SMTFEDTSNF
    YHPNFPFSGK IRVRGHDDSF LKNHLVFLVI YGTNGTFNQT LVTDNNGLAP
    FTLETSGWNG TDVSLEGKFQ MEDLVYNPEQ VPRYYQNAYL HLRPFYSTTR
    SFLGIHRLNG PLKCGQPQEV LVDYYIDPAD ASPDQEISFS YYLIGKGSLV
    MEGQKHLNSK KKGLKASFSL SLTFTSRLAP DPSLVIYAIF PSGGVVADKI
    QFSVEMCFDN QVSLGFSPSQ QLPGAEVELQ LQAAPGSLCA LRAVDESVLL
    LRPDRELSNR SVYGMFPFWY GHYPYQVAEY DQCPVSGPWD FPQPLIDPMP
    QGHSSQRSII WRPSFSEGTD LFSFFRDVGL KILSNAKIKK PVDCSHRSPE
    YSTAMGAGGG HPEAFESSTP LHQAEDSQVR QYFPETWLWD LFPIGNSGKE
    AVHVTVPDAI TEWKAMSFCT SQSRGFGLSP TVGLTAFKPF FVDLTLPYSV
    VRGESFRLTA TIFNYLKDCI RVQTDLAKSH EYQLESWADS QTSSCLCADD
    AKTHHWNITA VKLGHINFTI STKILDSNEP CGGQKGFVPQ KGRSDTLIKP
    VLVKPEGVLV EKTHSSLLCP KGKVASESVS LELPVDIVPD STKAYVTVLG
    DIMGTALQNL DGLVQMPSGC GEQNMVLFAP IIYVLQYLEK AGLLTEEIRS
    RAVGFLEIGY QKELMYKHSN GSYSAFGERD GNGNTWLTAF VTKCFGQAQK
    FIFIDPKNIQ DALKWMAGNQ LPSGCYANVG NLLHTAMKGG VDDEVSLTAY
    VTAALLEMGK DVDDPMVSQG LRCLKNSATS TTNLYTQALL AYIFSLAGEM
    DIRNILLKQL DQQAIISGES IYWSQKPTPS SNASPWSEPA AVDVELTAYA
    LLAQLTKPSL TQKEIAKATS IVAWLAKQHN AYGGFSSTQD TVVALQALAK
    YATTAYMPSE EINLVVKSTE NFQRTFNIQS VNRLVFQQDT LPNVPGMYTL
    EASGQGCVYV QTVLRYNILP PTNMKTFSLS VEIGKARCEQ PTSPRSLTLT
    IHTSYVGSRS SSNMAIVEVK MLSGFSPMEG TNQLLLQQPL VKKVEFGTDT
    LNIYLDELIK NTQTYTFTIS QSVLVTNLKP ATIKVYDYYL PDEQATIQYS
    DPCE
    2. Features
    INTENDED USE
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of a2ML1 in human serum, plasma, tissue homogenates and other biological fluids.
    DETECTION RANGE
    0.156-10ng/mL. The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/m,0.625ng/mL, 0.312ng/mL, 0.156ng/mL.

    SENSITIVITY
    The minimum detectable dose of a2ML1 is typically less than 0.067ng/mL.
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    SPECIFICITY
    This assay has high sensitivity and excellent specificity for detection of a2ML1.
    No significant cross-reactivity or interference between a2ML1 and analogues was observed.
    Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between a2ML1 and all analogues, therefore, cross reactivity may still exist.

    IMPORTANT NOTES
    1. Limited by the current conditions and scientific technology, it is impossible to conduct comprehensive identification and analysis tests on the raw materials provided by suppliers. As a result, it is possible there are some qualitative and/or technical risks.
    2. The final experimental results will be closely related to the validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available to obtain accurate results.
    3. Kits from different batches may be a little different in detection range, sensitivity and color developing time. Please perform the experiment exactly according to the instruction manual included in your kit. Electronic ones on our website are for reference only.
    4. Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    5. Protect all reagents from strong light during storage and incubation. All bottle caps of reagents should be closed tightly to prevent evaporation of liquids and contamination by microorganisms.
    6. There may be a foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    7. Incorrect procedures during reagent preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    8. Even the same experimenter may get different results from two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before the general assay for each batch is recommended.
    9. Each kit has undergone several rigorous quality control tests. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipment. Intra-assay variance among kits from different batches could arise from the above factors as well.
    10. Kits from different manufacturers with the same item might produce different results, since we have not compared our products with other manufacturers.
    11. The standard of the kit and immunogen used for antibody preparation are commonly recombinant proteins. Different expressed sequence, expression systems, purification methods might be used in recombinant protein preparation. Besides, there might exist differences on the screening technique of antibody and antibody pairs in our kit. Thus we can not guarantee the kit could detect recombinant protein from other companies. So, it is not recommended to use the kit for the detection of recombinant protein.
    12. Validity period: 12 months.
    13. The instruction manual also works with the 48T kit, but all reagents in the 48T kit are reduced by half.

    PRECAUTION
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.

    You can reference link of the kit as following
    http://www.dldevelop.com/uploadfile/data/DL-a2ML1-Hu.pdf
    Introduction
    ItemStandardTest
    Description

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of a2ML1 in human serum, plasma, tissue homogenates and other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120μLDetection Solution A 1×12mL
    Detection Reagent B 1×120μLDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mL
    Instruction manual 1Instruction manual 1
    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to a2ML1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to a2ML1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain a2ML1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of a2ML1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Recovery

    Matrices listed below were spiked with certain level of recombinant a2ML1 and the recovery rates were calculated by comparing the measured value to the expected amount of a2ML1 in samples.

    MatrixRecovery range (%)Average(%)
    serum(n=5)87-10194
    EDTA plasma(n=5)90-10195
    heparin plasma(n=5)91-10296
    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of a2ML1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample1:21:41:81:16
    serum(n=5)82-96%83-98%81-99%93-101%
    EDTA plasma(n=5)88-101%86-95%90-102%80-93%
    heparin plasma(n=5)80-91%82-90%95-104%79-95%






































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