Canine Babesiosis Pcr Kit EDTA Nucleic Acid Testing Kit PCR Fluorescent

Canine Babesiosis (B.C) Nucleic acid testing kit

(PCR-Fluorescent Probe Method)

Intended use:

This kit is suitable for the specific detection of Babesiosis (B.C) in canine EDTA whole blood and other samples.

Principle:

This kit uses the conserved gene fragment of Canine Babesiosis (B.C) to design a specific probe. The probe can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of the Taq enzyme will 5 The '-end fluorescent group is cleaved from the probe, making it free in the reaction system, thereby detaching from the 3'-end quenching group and emitting fluorescence, which is detected by a fluorescence PCR instrument to realize the detection of Canine Babesiosis (B.C) in a fully closed reaction system. Detection of Beth worm nucleic acid.

Main components:

Sample requirements:

1. Sample type

Canine EDTA whole blood etc.

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

Method calibration:

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM is selected for detection channels.

Positive judgment value or reference interval

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

Operation details please check from IFU!

Bio key Health is a high-tech enterprise with integrating R&D, production and sales&marketing in Guangzhou. Since 2003. Bio Key was founded by the "Thousand Talents Program" entrepreneurial team and the overseas senior automation equipment development engineer team.

The company's core team has been in the field of IVD research and entrepreneurship for more than 10 years, and has mastered a wealth of molecular diagnostic POCT industrial application technology, SmartTRF immune detection technology, RT qPCR nucleic acid detection technology and independent research and development capabilities of instruments.

It has a number of independent core technologies in the fields of automation engineering, micro-precision manufacturing, immunofluorescence, nucleic acid detection and gene sequencing. Since its establishment, the company has applied for more than 60 patents and currently has 17 authorized patents. The company has two R&D centers for diagnostic reagents and instruments and equipment, totaling more than 500 square meters; it has established more than 2,000 square meters of GMP workshops and equipment production plants; it has SmartTRF new immune detection technology, nucleic acid detection technology (POCT) and three scientific services.